By: Sherif Edris, PhD
Consultant
Lecturer
Molecular Cytogenetic Laboratory – Ain Shams University
Research Staff
Environmental stress laboratory
Genomics & Proteomics Laboratory
Agricultural Genetics Engineering Researching Institute (AGERI)
P.T Staff Member
Biology department
American university in Cairo (AUC)

Diploma targets candidates with Molecular biology interests, academic backgrounds, or work/researching experience.
Course includes a 30-hour-practical component in a laboratory environment hosted at one of our Research Partners (National Research Center, National Cancer Center, and others…) on
(a 5-hour-session-basis (6 consecutive full days, or once a week))

 

• Theoretical Component (60 Hours):
• DNA as genetic material
• Structure of DNA and RNA
• Bacterial restriction/modification system
• DNA modifying enzymes
• Introduction to Prokaryotic DNA replication
• Prokaryotic DNA replication, cont.
• DNA super coiling
• DNA supercoiling, cont., Topoisomerases
• Introduction to bacteria

Exam #1

 

• Introduction to bacteria, cont.
• Bacterial reproduction and growth
• Bacterial growth, cont
• Extrachromosomal elements, plasmids, selectable markers
• Central Dogma, genetic code
• Gene and operon
• lac operon, CAP site
• lac operon, cont. DNA footprinting
• Transcriptional regulation, transcription termination, the trp operon
• MRNA translation
• Gel electrophoresis

Exam #2

• DNA sequence analysis
• Polymerase chain reaction (PCR)
• PCR, cont
• Cloning PCR products
• Prokaryotic expression vectors
• Protein Sequencing, Peptide Mapping, Synthetic Genes
• cDNA libraries
• Genomic libraries
• Protein purification: assays, initial steps, resins

Exam #3

• Protein Purification: Ion exchange elution, dialysis, concentration
• Protein purification: types of resins, cont., plumbing
• Protein purification: running the experiment, resolving peaks
• M13 bacteriophage
• Phage display libraries, bacterial display, DNA binding protein display
• SELEX system
• Protein-Protein Recognition Probed Using a Yeast Transcriptiona
• Activator System
• Molecular Imprinting

Exam #4

 

B. Practical Component (30 hours) :
The lab-work serves three main functions:
• Students obtain practical experience in applications of basic techniques in molecular biology and genetic Engineering, including:

• DNA isolation from different sources
• DNA amplification (PCR)
• Construction of recombinant DNA
• Bacterial transformation
• Proofing of transformation and construction
• Prokaryotic transformation
• RT-PCR
• Southern blotting
• DNA sequencing
• Protein isolation
• Protein electrophoresis
• Isozyme electrophoresis
• Protein purification

Students learn to write laboratory reports based on research data and theoretical background.
The laboratory experience serves to aid in the Understanding of the materials covered in lectures.

 

Duration:90 Hours

Cost:2490 LE